杭州浩克生物
Product Information
| Description | ZO1 tight junction protein rabbit polyclonal |
| Protein full name | Tight junction protein ZO-1 |
| Synonyms | TJP1, ZO-1, Tight junction protein 1, TJP1, Zonula occludens protein 1 |
| Immunogen | KLH conjugated Synthetic peptide corresponding to Human ZO-1 |
| Isotype | IgG |
| Purity | Affinity purification |
| Subcellular location | Cell membrane |
| Predicted MW. | 195 kDa |
| Observed MW. | 220 kDa |
| Uniprot ID | Q07157, P39447 |
Applications
| Applications | WB,IF |
| Species | Human, Mouse,Human, Mouse, Ra |
| Dilution | 1: 500-1: 1000,1: 500-1: 1000 |
| Positive Tissue | kidney, spleen,kidney cancer, jejunum, small intestine |
Background
Tight junction (or zonula occludens) form the continuous intercellular barrier between epithelial and endothelial cells, which is required to separate tissue spaces and regulate selective movement of solutes across the epithelium and endothelium. ZO-1 is a peripheral membrane phosphoprotein located on the cytoplasmic face and is expressed in tight junctions of both epithelial and endothelial cells . It binds the transmembrane proteins occludin and the claudins linking them to cytoskeletal actin . ZO-1 belongs to a family of multidomain proteins known as the membrane-associated guanylate kinase homologs (MAGUKs). It is a pivotal tight junction protein and may be involved in signalling mechanisms regulating cell proliferation and differentiation.
Images
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Western blot analysis of ZO-1 (GB111402) at dilution of 1: 1000 Lane 1: HEK293 cell lysate Lane 2: HT29 cell lysate Lane 3: HepG2 cell lysate Lane 4: MCF7 cell lysate Lane 5: A431 cell lysate Lane 6: Mouse kidney tissue lysate Lane 7: Mouse spleen tissue lysate |
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Immunofluorescent analysis of paraformaldehyde-fixed human kidney cancer using ZO-1 (GB111402) at dilution of 1: 800 |
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Immunofluorescent analysis of paraformaldehyde-fixed mouse jejunum using ZO-1 (GB111402) at dilution of 1: 800 |
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Immunofluorescent analysis of paraformaldehyde-fixed mouse small intestine using ZO-1 (GB111402) at dilution of 1: 800 |
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Immunofluorescent analysis of paraformaldehyde-fixed rat small intestine using ZO-1 (GB111402) at dilution of 1: 800 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE: This product is intended for research only.
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